Introduction to Buchnera
Genome analysis
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Protocols
Comparative genomics
Proteome analysis
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Buchnera sp. APS is an
endocellular bacterial symbiont harboured by pea aphids, Acyrthosiphon
pisum. Buchnera belongs to the gamma-Proteobacteria, closely related
to the Enterobacteriaceae family. The mutualism between Buchnera
and its host is so obligate that neither associant can independently reproduce.
The first for symbiotic bacteria,
we determined the complete genome sequence of Buchnera sp. APS by
the whole genome random sequencing method (Shigenobu et al.
Nature 407, 81-86 (2000)).
The genome of Buchnera
sp. APS is comprised of one 640,681 base pair chromosome and two small
plasmids. Our analysis of the genome revealed the characteristic gene repertoire
of Buchnera, which is quite different from those of free-living
and parasitic bacteria. The characteristic gene set shows the tight mutualism
between Buchnera and the aphid hosts at the genomic level.
In this website, we provide the genome
data of Buchnera sp. APS in the comprehensive framewarks. In addition,
We will link the genome data to the ongoing our studies, the comparative
genomic analysis and the proteome analysis. |
Electron micrograph of Buchnera sp. APS
Photo by M. Morioka
The host aphids, Acyrthosiphon pisum (Harris)
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